Biochem/physiol Actions

Reversible: yes

Product competes with ATP.

Target IC₅₀: 7 nM, 20 nM, 1.3 nM, 700 pM, and 8.5 nM against protein kinase A , CaM kinase, myosin light chain kinase, protein kinase C, and protein kinase G, respectively

Cell permeable: yes

Primary TargetPKA

General description

A potent, cell-permeable, reversible, ATP-competitive and broad spectrum inhibitor of protein kinases. Inhibits protein kinase A (IC₅₀ = 7 nM), CaM kinase (IC₅₀ = 20 nM), myosin light chain kinase (IC₅₀ = 1.3 nM), protein kinase C (IC₅₀ = 700 pM), and protein kinase G (IC₅₀ = 8.5 nM). Also inhibits platelet aggregation induced by collagen or ADP but has no effect on thrombin-induced platelet aggregation. Induces apoptosis in human malignant glioma cell lines. Arrests normal cells at the G₁ checkpoint. A 1 mM (100 µg/214 µl) solution of Staurosporine (Cat. No. 569396) in DMSO is also available.

A potent, cell-permeable, reversible, ATP-competitive and broad spectrum inhibitor of protein kinases. Inhibits CaM kinase (IC₅₀ = 20 nM), myosin light chain kinase (IC₅₀ = 1.3 nM), protein kinase A (IC₅₀ = 7 nM), protein kinase C (IC₅₀ = 0.7 nM), and protein kinase G (IC₅₀ = 8.5 nM). Also inhibits platelet aggregation induced by collagen or ADP but has no effect on thrombin-induced platelet aggregation. Induces apoptosis in human malignant glioma cell lines. Arrests normal cells at the G₁ checkpoint.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Couldwell, W.T., et al. 1994. FEBS Lett.345, 43.Nishimura, H. and Simpson, I.A. 1994. Biochem. J. 302, 271.Bruno, S., et al. 1992. Cancer Res.52, 470.Kiss, Z. and Deli, E. 1992. Biochem. J. 288, 853.Vitale, M.L., et al. 1992. Neuroscience51, 463.Hoffman, R. and Newland, E.S. 1991. Cancer Chemother. Pharmacol.28, 102.Oka, S., et al. 1986. Agric. Biol. Chem.50, 2723.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

100, 250 µg in Plastic ampoule

Preparation Note

Further dilute with aqueous buffers just prior to use.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.

Warning

Toxicity: Standard Handling (A)